The responsibility for requesting a laboratory service or test lies with an authorised and trained practitioner (normally a Clinician). It is the responsibility of the requester to ensure that the identity of the patient is confirmed and that all samples are correctly labelled and request forms are completed to agreed standards in accordance with the Laboratory Medicine Sample Acceptance Procedure, failure to do so may result in the sample being rejected.
Urgent requests
Procedure for requesting urgent tests are located within departmental handbooks, located on their respective webpage.
Preparation for the patient and consent requirements
Certain tests require patient consent to be given due to the nature of the testing and the consequences of the results. Any genetics testing requested must have the patient’s informed consent prior to taking the sample. When the electronic order is generated, it is understood that the Doctor named on the form has discussed the consequences of the results with the patient and obtained consent. Any manual forms must be signed by the Doctor to indicate that consent has been obtained. Any special considerations for the preparation of a patient or requirements for the sample are provided in the departmental handbooks located on the department’s webpage. Please refer to the information in the handbooks prior to taking samples.
Specimen collection
Before collecting patient samples please refer to the department user handbook for information on the type and amount of the sample required and the sample container including any additives if required. The handbook will also outline any special timings for the sample and instructions for the inclusion of clinical information relevant to or affecting sample collection, test performance or result interpretation (e.g. history of administration of drugs, fasting sample).
Blood samples must be collected in line with the local Venepuncture procedure. The person collecting the sample should be identified on the request form and sample.
Order of draw
- Native blood
- Citrate blood
- Heparin blood
- EDTA blood
- Fluoride blood
The table below outlines the different types of anticoagulant and sample volume requirements for the tests listed in the test repertoire table. The information in the test repertoire table refers to 1 x adult or 1 x paediatric sample unless stated otherwise.
Colour reference in test repertoire table | Anticoagulant used | Standard bottle volume (Adult) | Paediatric bottle volume |
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Red | K-EDTA (Potassium-Ethylenediaminetetraacetic acid | 4.0ml | 1.2ml |
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Brown | None. Gel used to separate red cells from serum. | 4.9ml | 1.1ml |
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Green | Trisodium citrate | 4.3ml | 1.4ml |
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Yellow | Fluoride | 4.9ml or 2.7ml | 1.1ml |
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Orange | Lithium Heparin | 9.0ml | 1.1ml |
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Please note that any deviation from the established collection procedures may result in potential rejection of samples. Where the sample is deemed as precious, testing may still be performed but additional comments will be added to the result to communicate the potential risk from sample deviations.
Completing the request form
Samples for Blood Sciences and Microbiology can be requested using electronic ordercomms systems (Meditech and CiniSysICE). The use of electronic ordercomms reduces the number of sample rejections as it prompts for mandatory information required by the laboratory to carry out testing and it also speeds up the sample receipt process.
Handwritten request forms must comply with the Laboratory Medicine Sample Acceptance Procedure.
Requesting the clinician and location
Please ensure that the clinician and requesting location are completed and legible on the handwritten request form. Failure to do so may lead to a delay in processing.
Please note that abbreviations may result in the incorrect clinician or location being selected and as a consequence, the results for the patient may be sent back to another clinician.
Whilst the laboratory staff make every effort to minimise errors, staff can only book samples into the system with the information they are provided with.
Clinical information
Clinical details should also be included on the request. These can be significant to the results obtained and, in some cases, insufficient clinical details may mean that the request is rejected.
'Danger of infection' stickers
Samples from patients with blood borne virus diseases constitute a particular hazard to laboratory staff. All infectious or potentially infectious specimens and their accompanying request forms should be clearly marked with 'Danger of Infection' stickers. The range of investigations available on such specimens may be limited. Please contact the laboratory for further information.